Dr. Harinder Singh

Assistant Professor,

Biological Sciences
B.Sc. (Hons.)-Microbiology, M.Sc.-Biotechnology, Ph.D.-Microbiology

Areas of Specialization

Molecular Biology, Biochemistry and Microbiology

Academic/ResearchExperience

Dr. Harinder has been working with Sunandan Divatia School of Science from June 2016 and has over 19 years of research/teaching experience, which includes over 12 years post Ph. D work. He obtained his Ph.D. in Microbiology from Bhabha Atomic Research Centre, Mumbai, India, where he worked in the area of cyanobacterial stress response. He worked in a private company as Research Scientist immediately after his Ph.D. Later, he worked as postdoctoral researcher at Korean Atomic Energy Research Institute, South Korea and then at IIT Bombay, Mumbai, India. He had also worked as an Assistant Professor in an undergraduate college before obtaining his Ph.D.

Current Academic and non-Academic Activities

·Teaching various subjects like Biochemistry, Microbiology, Bioinformatics, R and Python, to B.Sc., M.Sc., Integrated M.Sc.-Ph.D. and Ph.D. students of Biomedical Science and Biological Sciences

·Program Chairperson - M.Sc./Integrated M.Sc.-Ph.D./Ph.D. Biological Sciences

·Member Secretary: BOS of the "M.Sc./Integrated M.Sc.-Ph.D./Ph.D. Biological Sciences” program.

·Guiding research work of 4 Ph. D. students

·Guest Editor, MDPI Pathogens

·Guest Editor, for JoVE (Methods Collection)

·BOS Member, Zoology, Mithibai College, Mumbai

·BOS Member, Botany, St. Xavier’s College, Mumbai

·BOS Member, Microbiology, Mithibai College, Mumbai

·Reviewer for various journals like Frontiers in Microbiology, DNA Repair, Extremophiles, Molecular Biotechnology, Microbial Ecology, Journal of Medical Microbiology, Molecular and Cellular Toxicity, Biofouling, Food Science and Technology, Freshwater Biology, Soil Biology and Biochemistry.

Membership / Fellowship of Professional Societies

• Life member of Association of Microbiologist of India (AMI)
• Life member of Biotech Research Society, India (BRSI)

Area of Research

Stress biology, Enzyme engineering, Oral Cancer, Fatty acid production. For more details, please visit:

https://science.nmims.edu/faculty-and-research/research-areas-and-groups/dr-harinder-singh/

Publications

Year 2020-22

• Kamble A, Singh H. (2022). Finding novel enzymes by in silico bioprospecting approach. In: Value-Addition in Food Products and Processing Through Enzyme Technology. Ed: M Kuddus and CN Aguilar. Pp 347-364.
• Cardoza E, Singh H. (2022). Involvement of CspC in response to diverse environmental stressors in Escherichia coli. Journal of Applied Microbiology 132 (2), 785-801.
• Sawant N, Singh H, Appukuttan D. (2022). Overview of the Cellular Stress Responses Involved in Fatty Acid Overproduction in E. coli. Molecular Biotechnology. 64, 373-387.
• Sawant S, Dugad J, Parikh D, Srinivasan S, Singh H. (2021) Identification & correlation of bacterial diversity in oral cancer and long- term tobacco chewers- A case- control pilot study. Journal of Medical Microbiology. 70, 001417
• Cardoza E, Singh H. (2021) C Group-Mediated Antibiotic Stress Mimics the Cold Shock Response. Current Microbiology. 78 (9), 3372-3380..
• Sawant S, Dugad J, Parikh D, Singh H. (2021) Absolute quantitation of oral bacteria involved in oral cancer by real-time PCR. Medicine in Microecology. Accepted 24^th March, 2021.
• Nagar A, Kamble A, Singh H. (2021) Preliminary screening, isolation and identification of microbial phytase producers from soil. Environ Exp Biol 19: 11–22
• Kamble A, Singh H. (2020) Different Methods of Soil DNA Extraction.Bio-101. e3521
• Kamble A, Sawant S, Singh H. (2020) 16S ribosomal RNA gene-based metagenomics: A review. Biomedical Research Journal. 7(1):5

Year 2017-19

• Kamble A, Srinivasan S, Singh H. (2019) In-Silico Bioprospecting: Finding Better Enzymes. Molecular Biotechnology. 61(1):53-59.
• Singh H, Apte SK. (2018) Effect of 60Co-gamma ionizing radiation and desiccation stress on protein profile of Anabaena 7120. The Protein Journal. 37(6):608-621.
• Singh H. (2018) Desiccation and radiation stress tolerance in cyanobacteria. Journal of Basic Microbiology. 58 (10), 813-826.
• Shah S, Agera R, Sharma P, Sunder AV, Singh H, et al. (2018) Development of biotransformation process for asymmetric reduction with novel anti-Prelog NADH-dependent alcohol dehydrogenases. Process Biochemistry. 70, 71-78. Impact Facor-2.6

• Singh H, Apte SK. (2018) Low concentrations of ethanol during irradiation drastically reduce DNA damage caused by very high doses of ionizing radiation. Journal of Biosciences. 43 (1), 15-23.
• Park S-H, Singh H, et al. (2017) PprM, a Cold Shock Domain-Containing Protein from Deinococcus radiodurans, Confers Oxidative Stress Tolerance to Escherichia coli. Front. Microbiol. 7: 2124. Impact Facor-4.0

Till 2016

• Appukuttan D, Singh H, et al. (2016) Engineering Synthetic Multistress Tolerance in Escherichia coli by Using a Deinococcal Response Regulator, DR1558. Appl Environ Microbiol. 82(4):1154-66. Impact Facor-3.6
• Singh H, Appukuttan D, Lim S. (2014) Hsp20, a small heat shock protein of Deinococcus radiodurans, confers tolerance to hydrogen peroxide in Escherichia coli. Journal of Microbiology and Biotechnology 24(8):1118-22.
• Singh H, et al. (2013) High radiation and desiccation tolerance of nitrogen-fixing cultures of the cyanobacterium Anabaena sp. Strain PCC 7120 emanates from genome/proteome repair capabilities. Photosynthesis Research 118: 71-81. Impact Facor-3.0

For full list of publication, please visit:

https://scholar.google.co.in/citations?user=vu5tFz4AAAAJ&hl=en&oi=sra

Conference Proceedings

1. Singh H, et al. (2012). A Novel Radiation-Resistant Yeast, Filobasidium elegans RRY1. In Proceedings of: Transaction of The Korean Nuclear Society Spring Meeting. Jeju, South Korea, 793-94.

2.Singh H, et al. (2004). Radioresistance of nitrogen fixing Cyanobacterium anabaena sp PCC 7120. Indian Journal of Radiation Research, 1(2), 63-64.

Posters Presented In Conferences/Symposia/Meetings

1.Kamble A, Singh H. (2019) Computational analysis of putative phytase enzymes. International Conference on Advances in Materials Science & Applied Biology (AMSAB). Jan 8–10th. Mumbai, India.

2.Khakhar H, Singh H, Chandra S. (2019) Assessment of in vitro toxicity through MTT assay of biocompatible gold nanoparticles to be used for photothermal therapy (PTT) on different cell line. International Conference on Advances in Materials Science & Applied Biology (AMSAB). Jan 8–10th. Mumbai, India.

3.Priyanka, Singh H, et al. (2015) Exploring evolutionary diversity to identify putative Alcohol dehydrogenase enzymes as starting point for directed evolution. Enzyme Engineering XXIII. September 6-11, St. Petersburg, Florida, USA.

4.Wangikar PP, Singh H, et al. (2015) Engineering of Ketoreductase Enzymes For Chiral Synthesis On Industrial Scale. Enzyme Engineering XXIII. September 6-11, St. Petersburg, Florida, USA

5.Singh H, et al. (2015) Novel Alcohol Dehydrogenase Enzyme for Chiral Synthesis. BIOTRANS 2015, Jul 26-30, Vienna, Austria.

6.Wangikar PP, Singh H, et al. (2015) Cofactor Recycling System For Enzyme Mediated Asymmetric Synthesis. BIOTRANS 2015, July 26-30, Vienna, Austria.

7.Deepti H, Singh H, et al. (2013) Enhanced stress tolerance of Escherichia coli harboring a response regulator of Deinococcus radiodurans. Bioenergy Korea Conference & The 9^th KSMB Annual Meeting International Symposium, 12-14 Nov, Lotte Hotel, Jeju, South Korea.

8.Deepti H, Singh H, et al. (2013) Role of Essential Response Regulator in Maintaining Homeostasis of Deinococcus radiodurans. 40th Anniversary International Symposium & Annual Meeting (ISAM), The Korean Society for Microbiology and Biotechnology (KSMB), 3-5 July, Alpensia, Pyeongchang, South Korea.

9.Singh H, et al. (2013) The effect of Deinococcal Hsp20 on Oxidative Stress Tolerance of Escherichia coli. 40th Anniversary ISAM, KSMB, 3-5 July, Alpensia, Pyeongchang, South Korea.

10.Deepti H, Singh H, et al. (2012) Involvement of two-component system in stress tolerance of Deinococcus radiodurans. Korean Society for Molecular and Cellular Biology (KSMCB) Annual Meeting, 10-12 October, COEX, Seoul, South Korea

11.Singh H, et al. (2012) Effect of the Deinococcus radiodurans hsp20 and pprM on the Resistance of Escherichia coli to DNA Damaging Agents. ISAM, KSMB, 27-29 June, BEXCO, Busan, South Korea.

Conference / Seminars / Workshop Attended

·Five-day online Faculty Development Program on 'Precision Medicine', 19^th – 23^rdJanuary 2021, at School of Biotechnology and Bioinformatics (DYPUNM-SBB), D Y Patil Deemed to be University, Navi Mumbai, Maharashtra.

·Two-Day Faculty Development Programme On LaTeX for Mathematics and Research Documentation, 10 - 11 Jan 2020, at School of Technology Management and Engineering, SVKM's NMIMS, Navi Mumbai, Maharashtra.

·Committee Member for Organizing International Conference on Advances in Materials Science & Applied Biology. 8–10^th January 2019. Mukesh Patel Auditorium, SVKM's NMIMS (Deemed to be University), Mumbai, Maharashtra.

·Pedagogy Workshops for Undergraduate Science Teachers, February 27-1 March, 2017, at IISER Pune, Pune, Maharashtra

·One-day seminar on "Chromatography and Mass Spectrometry: An Industry Perspective”, 1st October, 2016, at Dr. Bhanuben Nanavati College of Pharmacy, Mumbai, Maharashtra.

·Indo-US workshop on "Cell Factories”, 18-20 March, 2016, at IIT Bombay, Mumbai

·One-day workshop on "Advances in Algal Biotechnology" on 21st November, 2015, at IIT Bombay, Mumbai, Maharashtra

Other Qualifications/Achievements/Fellowships

• 2003-08: Junior & Senior research fellowship from Council of Scientific and Industrial Research (CSIR)
• 2002: Qualified CSIR-UGC NET-JRF (National Eligibility Test, Junior Research Fellowship)
• 2002: Qualified ICMR-JRF (Indian Council of Medical Research-Junior Research Fellowship)
• 2000-02: M.Sc. Scholarship from DBT (Department of Biotechnology), Government of India
• 1999-2000: One-year Post Graduate Diploma in Bio-Chemical Technology, University of Delhi, India

Official Email Id

Harinder.Singh@nmims.edu

Official Phone No.

022-4235-5938