A five day Cell Culture and Molecular Biology workshop was conducted from 11-15th January 2016 at Sunandan Divatia School of Science (SDSOS). This course was designed as a stepping stone to educate and train participants who were new to mammalian cell culturing systems or as a refresher course in cell culture and molecular biology techniques. The workshop was second in the series and included a guest talk, informal lectures and hands-on sessions. The guest speaker Dr. Pallavi Karnik, Principal Scientist, Biogenomics Limited gave a talk on “Cell culture and its applications: An Industrial Perspective”. This was followed by talk by Dr. Purvi Bhatt, Assistant Professor, SDSOS. The contents of the workshop were as follows: Cell culturing techniques; Maintenance and subculturing of cells; Preservation and cell revival; Cytotoxicity assays (MTT assay); Cell death Assays: based on morphology (Acridine orange/Ethidium bromide staining) and based on Molecular Techniques (Western Blot, DNA laddering); Real time PCR & Immunofluorescence staining. There was an overwhelming response to attend this workshop and in all there were 16 participants from Academia/Hospitals/Industry: Sir HN Hospital, Nair Hospital, Hinduja Hospital, Accutest Global, SPP-SPTM, BNCP, Thakur college to name a few. The workshop was partially supported by Eppendorf, Chargen Life Sciences, Bhavi Chem and C. Abhaykumar & Co. Ltd. The students were greatly benefitted and appreciated the five day workshop.